1/16/2024 0 Comments Helicon focus with gimp![]() We can now combine the images using enfuse. The -a switch allows you to set a prefix for the output images (otherwise they will just over-write the originals), -v means "verbose output messages" (not necessary, but allows you to watch progress as it runs) and -m tells it to correct the magnification of all but the first image (otherwise it only considers x,y shifts and rotations). This will probably take a minute or two and result in a set of files called align_ nnnn.tif in a subdirectory \work from the directory where the original image files were located. "align_image_stack.exe" -a "\work\align_" -m -v As we move the camera nearer and further from the specimen, or change the focus of the lens, to cover the different focal planes, we inevitably change the magnification slightly.Īssuming that the \Hugin\bin directory is in your PATH environment variable (so that Windows can find Hugin's executable files) and that you are running the command from the directory where the TIFF images are located, the command will look like this:
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